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KMID : 0043320040270121253
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Archives of Pharmacal Research
2004 Volume.27 No. 12 p.1253 ~ p.1257
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Apoptosis Inducing Effects of 6-Methoxydihydrosanguinarine in HT29 Colon Carcinoma Cells
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Lee Yong-Jin
Yin Hu-Quan
Kim Young-Ho
Li Guang-Yong
Lee Byung-Hoon
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Abstract
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6-Methoxydihydrosanguinarine (6ME), a benzophenanthridine alkaloid derived from the methanol extracts of Hylomecon hylomeconoides, showed a dose-dependent effect at 1-10 on causing apoptotic cell death in HT29 colon carcinoma cells . Treatment of HT-29 cells with 6ME resulted in the formation of internucleosomal DNA fragmentation. Treatment of the cells with 6ME caused activation of caspase-3, -8 and 9 protease and subsequent proteolytic cleavage of poly(ADP-ribose)polymerase. 6ME increased the expression of p53 and Bax and decreased the expression of Bid. These results indicate that p53 and proapoptotic Bcl-2 family proteins might participate in the antiproliferative activity of 6ME in HT29 cells.
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KEYWORD
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6-Methoxydihydrosanguinarine, Hylomecon hylomeconoides, Apoptosis, HT29 cells
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